| 產(chǎn)品名稱 |
MECA-367 |
| 商品貨號(hào) |
B165139 |
| Organism |
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody specifically blocks lymphocyte adhesion to mouse Peyer's patch endothelia and does not block lymphocyte binding to peripheral lymph nodes. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against mouse Peyer's patch endothelial cells (MAdCAM-1) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against mouse Peyer's patch endothelial cells (MAdCAM-1) |
| Comments |
Animals were immunized with mouse lymphnode stroma. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody specifically blocks lymphocyte adhesion to mouse Peyer's patch endothelia and does not block lymphocyte binding to peripheral lymph nodes. It recognizes the N terminal domain of mouse mucosal vascular addressin (MAdCAM-1) involved in alpha 4, beta 7 (a4b7) integrin binding. |
| Complete Growth Medium |
Culture Medium: RPMI 1640 medium, 90%; fetal bovine serum, 10%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgG2a |
| Name of Depositor |
Stanford University Board of Trustees |
| U.S. Patent Number |
|
| References |
Butcher EC. Method to control leukocyte extravasation. US Patent 5,403,919 dated Apr 4 1995
Streeter PR, et al. A tissue-specific endothelial cell molecule involved in lymphocyte homing. Nature 331: 41-46, 1988. PubMed: 3340147
Berlin C, et al. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell 74: 185-195, 1993. PubMed: 7687523
Briskin MJ, et al. MAdCAM-1 has homology to immunoglobulin and mucin-like adhesion receptors and to IgA1. Nature 363: 461-464, 1993. PubMed: 8502297
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