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CRI-O84
CRI-O84
規(guī)格:
貨期:
編號:B235039
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 CRI-O84
商品貨號 B235039
Designations CRI-O84
Species Homo sapiens, human
Vector
Construct size (kb): 46.0
Insert
DNA: genomic
Insert lengths(kb): 40.0
Gene product: DNA Segment, single copy [D16S43]
Alleles: B1, B1, B2, B2, B3, B4, A1, A2, B3, B4
Insert Size (kb) 40.0
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--19.0, 8.0, 5.5, 4.5, 2.5, 2.4, 2.2, 1.9; BamHI--17.0, 11.0, 7.0, 6.4, 3.1, 1.9; HincII--8.4 (doublet), 6.5, 3.3 (doublet), 3.0, 1.8, 1.75, 1.65, 1.4, 1.3;
HindIII--11.0, 6.5, 6.0, 5.4, 4.0 (doublet), 3.5, 2.1, 1.8; BglII--17.0 (doublet), 3.0 (doublet), 2.6.
The insert can be excised from the vector with EcoRI, resulting in a 5.5 kb EcoRI vector fragment.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
The PstI and HindIII polymorphisms have a combined PIC value of 0.79 and a combined heterozygosity of 83%.
The HindIII polymorphisms have a combined heterozygosity of 33%.
Enzyme(s) not detecting polymorphism: BglII, EcoRI, MspI (panel of 5 individuals).
References

Donis-Keller H, et al. A genetic linkage map of the huma genome. Cell 51: 319-337, 1987. PubMed: 3664638

Keith TP, et al. Genetic linkage map of 43 DNA markers on human chromosome 16. Proc. Natl. Acad. Sci. USA 87: 5754-5758, 1990. PubMed: 2377614

Jacobsen SJ, et al. A clinically homogeneous group of families with facioscapulohumeral (Landouzy-Dejerine) muscular dystrophy: linkage analysis of six autosomes. Am. J. Hum. Genet. 47: 376-388, 1990. PubMed: 1975474

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