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pG60/T10
pG60/T10
規(guī)格:
貨期:
編號(hào):B161879
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pG60/T10
商品貨號(hào) B161879
Designations pG60/T10
Species Saccharomyces cerevisiae Meyen ex E.C. Hansen
Depositors A Tzagoloff
Vector
Construct size (kb): 18.5
Insert
DNA: genomic
Insert lengths(kb): 6.699999809265137
Gene product: 5' end processing factor [CBP1]
Insert Size (kb) 6.700
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments
The insert contains the following sites separated by (kb) (approximate): BamHI- 0.8- BamHI- 0.6- BamHI- 0.7- HindIII- 2.8- BamHI.
Restriction digests of the clone give the following sizes (kb): EcoRI/SalI--7.8, 4.0, 3.5, 2.3, 0.9; XhoI--11.6, 7.0; XbaI--18.5; XbaI/AvaI--7.0, 3.5, 2.4, 2.0, 1.7, 0.6; BamHI--16.0, 2.5.
The insert includes a continuous open reading frame encoding a basic protein of 654 amino acids and 76 kDa. The mRNA is about 2.2 kb.
Classification Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
References

Dieckmann CL, et al. Assembly of the mitochondrial membrane system. Nucleotide sequence of a yeast nuclear gene (CBP1) involved in 5' end processing of cytochrome b pre-mRNA. J. Biol. Chem. 259: 4732-4738, 1984. PubMed: 6325407

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