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17aba
17aba
規(guī)格:
貨期:
編號(hào):B162227
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 17aba
商品貨號(hào) B162227
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Leukemia
Applications
The antibody reacts with human CD11b, and blocks all known CD11b dependent activities in vitro.
The antibody cross-reacts with CD11b from rat and canine cells.
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human CD11b
Cellular Products
immunoglobulin; monoclonal antibody; against human CD11b
Comments
Animals were immunized with human acute monocytic leukemia cells (FAM M6).
Spleen cells were fused with NS-1 myeloma cells.
The antibody reacts with human CD11b, and blocks all known CD11b dependent activities in vitro.
The antibody cross-reacts with CD11b from rat and canine cells.
Complete Growth Medium
Culture Medium: Modified Dulbecco's medium (see below), 90%; Gamma
globulin-free horse serum, 10%.
DME medium with 4.5 g/L glucose 100 mL
L-Glutamine (100x) 2 mL
Solution I 1 mL
Hypoxanthine (10 mM) - Thymidine (1.6 mM) 1 mL
NCTC 135 10 mL
Gamma Globulin-Free Horse Serum (inactivated) 12 mL
Solution I (100x)
1. 1320 mg oxalacetic acid (100 mM, MW 132).
2. 80 mg crystalline bovine insulin (20 units/mL, 25 units/mg).
Add 1 and 2, stir at 37°C. Add Na pyruvate 550 mg (50 mM, FW 110). Bring up to
100 mL with distilled water. Stir at 37°C until solution dissolves. Filter,
aliquot and store frozen.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgM; kappa light chain
Name of Depositor RF Todd
Deposited As mouse (B cell); mouse (myeloma)
References

. . J. Inflamm. 13: 495-505, 1989.

Dana N, et al. Two functional domains in the phagocyte membrane glycoprotein Mo1 identified with monoclonal antibodies. J. Immunol. 137: 3259-3263, 1986. PubMed: 2430017

Mulligan MS, et al. Roles of beta 2 integrins of rat neutrophils in complement- and oxygen radical-mediated acute inflammatory injury. J. Immunol. 148: 1847-1857, 1992. PubMed: 1347308

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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