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plambda500 [p500]
plambda500 [p500]
規(guī)格:
貨期:
編號:B162228
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 plambda500 [p500]
商品貨號 B162228
Designations plambda500 [p500]
Species Homo sapiens, human
Vector
Construct size (kb): 10.25
Insert
DNA: genomic
Insert lengths(kb): 5.900000095367432
Gene product: hypoxanthine phosphoribosyltransferase pseudogene 2 [HPRTP2]
Alleles: A1, A2
Insert Size (kb) 5.900
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA distributed as 5 ug in 50 ul TE (volume: 50 ul,
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--5.1 kb doublet; HindIII--10.25; PstI--10.25; EcoRI/BamHI--5.6, 4.1, 1.25; EcoRI/HindIII--5.1, 4.4, 0.9.
The 0.9 kb EcoRI/HindIII fragment represents the HPRTP2 gene.
The insert includes a 5.0 kb EcoRI fragment and a 0.9 kb EcoRI/HindIII fragment, with the latter subcloned from Hulambda5.
The 3.8 kb constant band is from the X chromosome.
Enzyme(s) not detecting polymorphism: TaqI, PstI, BstNI, XbaI, SacI, PvuII, HindIII, HincII, StuII, MboI, EcoRV.
References

Weiffenbach B, et al. A genetic linkage map of human chromosome 5 with 60 RFLP loci. Genomics 10: 173-185, 1991. PubMed: 1675192

Patel PI, et al. Organization of the HPRT gene and related sequences in the human genome. Somatic Cell Mol. Genet. 10: 483-493, 1984. PubMed: 6089358

C. Thomas Caskey, personal communication

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