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IgG-2F1
IgG-2F1
規(guī)格:
貨期:
編號:B162273
品牌:Mingzhoubio

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產(chǎn)品名稱 IgG-2F1
商品貨號 B162273
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Animals were immunized against GST-Rab escort protein-1 (REP-1).
The antibody is specific for REP-1 from human lymphoblastoid cell lines and peripheral blood leukocytes.
It is useful for detecting the level of REP-1. This antibody is used as a diagnostic tool for choroideremia (CHM), an X-linked form of retinal degeneration, by immunoblot analysis.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human REP-1
Cellular Products
immunoglobulin; monoclonal antibody; against human REP-1
Comments
Animals were immunized against GST-Rab escort protein-1 (REP-1).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody is specific for REP-1 from human lymphoblastoid cell lines and peripheral blood leukocytes.
It is useful for detecting the level of REP-1. This antibody is used as a diagnostic tool for choroideremia (CHM), an X-linked form of retinal degeneration, by immunoblot analysis.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Maintain cultures between 5 X 10 exp4 and 5 X 10 exp5 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype IgG1
Name of Depositor JL Goldstein, YK Ho
Deposited As mouse (B cell); mouse (myeloma)
References

MacDonald IM, et al. A practical diagnostic test for choroideremia. Ophthalmology 105: 1637-1640, 1998. PubMed: 9754170

Andres DA, et al. Mutational analysis of alpha-subunit of protein farnesyltransferase. Evidence for a catalytic role. J. Biol. Chem. 268: 1383-1390, 1993. PubMed: 8419339

Seabra MC, et al. Purification of component A of Rab geranylgeranyl transferase: possible identity with the choroideremia gene product. Cell 70: 1049-1057, 1992. PubMed: 1525821

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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