久久久久国产成人精品亚洲午夜,成人免费毛片一区二区三区,国产成人久久精品激情91,国产成人无码精品久久久免费,精品欧美国产一区二区三区不卡,国产成人一区二区三区影院,国产精品久久久久久,久久久WWW成人免费精品,欧美91精品久久久久网免费

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > CRE BAG 2
▍最近瀏覽歷史
▍聯(lián)系我們
  • 0574-87157013
  • [email protected]
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
CRE BAG 2
CRE BAG 2
規(guī)格:
貨期:
編號:B162306
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 CRE BAG 2
商品貨號 B162306
Organism Mus musculus, mouse
Tissue embryo
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease Leukemia
Strain NIH/Swiss
Applications
produces protein beta-galactosidase beta-D-galactosidase
Derivation
The CRE BAG 2 cell line has never produced a replication competent helper virus.
The cells will package vectors derived from murine leukemia viruses.
Genes Expressed
a beta galactosidase transducing vector (BAG),beta-galactosidase(beta-D-galactosidase) [lacZ],beta-galactosidase
Cellular Products
a beta galactosidase transducing vector (BAG)
Comments
The BAG construct has been used to transfect psiCRE cells in ATCC CRL-1858 and to transfect psi2 cells in ATCC CRL-9560.
The CRE BAG 2 cell line has never produced a replication competent helper virus.
This line produces a vector (BAG) that can infect mouse and rat cells and transduce beta-galactosidase activity.
The line is similar to the psi 2 BAG alpha line (see ATCC CRL-9560) but has never been known to produce a replication competent helper virus.
The cells will package vectors derived from murine leukemia viruses.
The vector has been used to infect a variety of neural cell types and to express beta-galactosidase and resistance to G418.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; bovine calf serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Remove medium, add fresh 0.25% trypsin - 0.02% EDTA and allow the flask to sit at room temperature (or 37C) until the cells detach (2 to 3 minutes). Add fresh medium, aspirate and dispense into new flasks.
Culture Conditions
Temperature: 37.0°C
Name of Depositor CL Cepko
References

Price J, et al. Lineage analysis in the vertebrate nervous system by retrovirus-mediated gene transfer. Proc. Natl. Acad. Sci. USA 84: 156-160, 1987. PubMed: 3099292

Turner DL, Cepko CL. A common progenitor for neurons and glia persists in rat retina late in development. Nature 328: 131-136, 1987. PubMed: 3600789

Danos O, Mulligan RC. Safe and efficient generation of recombinant retroviruses with amphotropic and ecotropic host ranges. Proc. Natl. Acad. Sci. USA 85: 6460-6464, 1988. PubMed: 3413107

Cepko CLVirus propagation and Xgal proceduresIn: Cepko CLNeuromethodsClifton, NJHumana Press1989.

Le Doux JM, et al. Proteoglycans secreted by packaging cell lines inhibit retrovirus infection. J. Virol. 70: 6468-6473, 1996. PubMed: 8709284

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
峨眉山市| 同江市| 建宁县| 临沂市| 澄迈县| 兴化市| 东辽县| 鄄城县| 阆中市| 民丰县| 宿迁市| 忻州市| 呈贡县| 崇文区| 武胜县| 顺义区| 扶沟县| 怀柔区| 巍山| 达尔| 洪洞县| 越西县| 光泽县| 乌拉特前旗| 沛县| 河津市| 靖宇县| 太康县| 大悟县| 武汉市| 色达县| 定南县| 大厂| 水富县| 诸暨市| 新乡县| 北宁市| 珠海市| 汶上县| 桦南县| 隆德县|