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pSL7
pSL7
規(guī)格:
貨期:
編號:B162372
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pSL7
商品貨號 B162372
Designations pSL7
Depositors T Lufkin
Biosafety Level 1
Vector Information
Size (kb): 6.4000000953674320
Vector: pSL7 (plasmid)
Construction: pBS
Marker(s):ampR
Construct size (kb): 6.400000095367432
Features: marker(s): ampR
other: internal ribosome entry sequence (IRES)
replicon: pMB1
reporter group: alkaline phosphatase
terminator: SV40 polyadenylation
Applications
reporter construct alkaline phosphatase, placental (Regan isozyme)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.1, 2.5, 0.6, 0.2; HindIII--5.5, 0.6, 0.3; SpeI--6.4.
Plasmid expressing alkaline phosphatase. Foreign genes can be cloned upstream of the IRES/reporter gene, allowing production of a dicistronic messenger RNA in mammalian cells.
IRES/reporter insert lacking polyA can be excised with NruI.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Li X, et al. Dicistronic lacZ and alkaline phosphatase reporter constructs permit simultaneous histological analysis of expression from multiple transgenes. BioTechniques 23: 874-882, 1997. PubMed: 9383553

Thomas Lufkin, personal communication

Shipped freeze-dried
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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