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pEBA-6
pEBA-6
規(guī)格:
貨期:
編號:B162394
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pEBA-6
商品貨號 B162394
Designations pEBA-6
Depositors EW Miles
Biosafety Level 1
Vector Information
Size (kb): 5.5399999618530270
Vector: pEBA-6 (plasmid)
Promoters: Promoter Ptrc
Construction: pCR2
Marker(s):TRP1,ampR
Construct size (kb): 5.539999961853027
Features: primer site: 5'-TTGACAATTAATCATCCGGC-3'
promoter: Ptrc
terminator: rrnBT1
terminator: rrnBT2
Applications
contains sequence
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.5; EcoRI--5.5; PstI--5.5; EcoRI/PstI--4.2, 1.4.
Overproduces the beta2 subunit of tryptophan synthase from Salmonella typhimurium.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Yang LH, et al. PCR mutagenesis and overexpression of tryptophan synthase from Salmonella typhimurium: on the roles of beta2 subunit Lys-382. Protein Expr. Purif. 8: 126-136, 1996. PubMed: 8812843

Jhee KH, et al. Domain architecture of the heme-independent yeast cystathionine beta-synthase provides insights into mechanisms of catalysis and regulation. Biochemistry 39: 10548-10556, 2000. PubMed: 10956046

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