久久久久国产成人精品亚洲午夜,成人免费毛片一区二区三区,国产成人久久精品激情91,国产成人无码精品久久久免费,精品欧美国产一区二区三区不卡,国产成人一区二区三区影院,国产精品久久久久久,久久久WWW成人免费精品,欧美91精品久久久久网免费

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > 8B1B.1
最近瀏覽歷史
聯系我們
  • 0574-87157013
  • [email protected]
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
8B1B.1
8B1B.1
規(guī)格:
貨期:
編號:B162400
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱 8B1B.1
商品貨號 B162400
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody is specific for the structural protein VP7.
Storage Conditions liquid nitrogen vapor phase
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against VP7 of bluetongue virus
Cellular Products
immunoglobulin; monoclonal antibody; against VP7 of bluetongue virus
Tumorigenic Yes
Effects
Yes, in BALB/c mice
Comments
Animals were immunized with bluetongue virus (serotype 17).
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody is specific for the structural protein VP7.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 6 x 104 viable cells/mL.  Maintain cultures at a cell concentration between 5 x 104 and 5 x 106 cells/mL.

Medium Renewal: Two to three times weekly
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype IgG2b
Name of Depositor JA House
Deposited As mouse (B cell); mouse (myeloma)
References

Appleton JA, Letchworth GJ. Monoclonal antibody analysis of serotype-restricted and unrestricted bluetongue viral antigenic determinants. Virology 124: 286-299, 1983. PubMed: 6186076

House C, et al. Detection of bluetongue group-specific antibody by competitive ELISA. J. Vet. Diagn. Invest. 2: 137-139, 1990. PubMed: 1965579

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479
威海市| 株洲市| 汽车| 扎鲁特旗| 汝阳县| 满洲里市| 阆中市| 平顺县| 通道| 比如县| 图木舒克市| 通河县| 启东市| 奉化市| 昌图县| 肇东市| 松溪县| 长顺县| 安陆市| 南陵县| 新昌县| 安仁县| 玉树县| 景东| 南宁市| 东阿县| 乐亭县| 阳谷县| 铁岭市| 剑河县| 波密县| 绵竹市| 虎林市| 西安市| 南涧| 云浮市| 桐柏县| 北辰区| 鸡泽县| 英德市| 工布江达县|