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Methanococcus maripaludis Jones et al.
Methanococcus maripaludis Jones et al.
規(guī)格:
貨期:
編號(hào):B162453
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) Methanococcus maripaludis Jones et al.
商品貨號(hào) B162453
Strain Designations LL [OCM 803]
Isolation
Intertidal sediments
Biosafety Level 1 WARNING: EXPLOSION HAZARD:

Methanogens that grow on methanol (a solute) produce methane gas resulting in over pressurization of growth vessels (3). This creates a potential explosion hazard. We recommend growing cultures in pressure-resistant Balch tubes* (see below) to reduce this risk. The cultures should be vented regularly to reduce the gas and prevent overpressure. If it is necessary to grow larger batches of methanol-utilizing methanogens in sealed serum vials, extra caution should be taken. Typically, 3 moles of methane are produced from one mole of methanol.

Always wear protective eye wear when working with methanogens growing in tubes or bottles.

*Balch tube refers to a special type of test tube that is designed to be pressurized and suited for anaerobic work. The Balch test tubes can be purchased from Bellco Glass (www.bellcoglass.com; stock no. 2048-00150).


Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions -80°C or colder
Preceptrol® no
Type Strain no
Comments
This item is part of The Oregon Collection of Methanogens (OCM) that was kindly donated to the ATCC by Dr. David Boone. This collection is now part of the ATCC general collection and available to the scientific community. OCM items are archived as original material and available on a "made-to-order" basis. ATCC will make a reasonable effort to recover these items from preservation, but recovery and identification is not guaranteed.
Medium ATCC® Medium 2469: MSH Medium
Growth Conditions
Temperature: 37°C
Atmosphere: Anaerobic gas mixture 80% H2 - 20% CO2
Name of Depositor D Boone
Special Collection Oregon Collection of Methanogens
References

Boone DR, et al. Diffusion of the interspecies electron carriers H2 and formate in methanogenic ecosystems and its implication in the measurement of Km for H2 or formate uptake. Appl. Environ. Microbiol. 55: 1735-1741, 1989.

Brockman F. Methanosarcina Culture Warning. ASM News 70: 47, 2004.

Sowers KR, Schreier HJ (Editors). Archaea: A laboratory manual, Cold Spring Harbor, NY: CSHL Press, 1995.

Cross References

Complete Genome (GenBank) : CP002913.1 Complete genome sequence

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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