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Op-30
Op-30
規(guī)格:
貨期:
編號:B162605
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Op-30
商品貨號 B162605
Designations Op-30
GenBank Number

X13694

Species Homo sapiens, human
Depositors MC Kiefer
Applications
The cell-binding sequence and a potential N-linked glycosylation site are conserved in both human and rat.
Vector
Construct size (kb): 4.0
Insert
DNA: cDNA
Insert lengths(kb): 1.399999976158142
Tissue: osteosarcoma
Gene product: secreted phosphoprotein 1 (includes osteopontin)( 44kDa bone phosphoprotein, Secreted phosphoprotein-1 (osteopontin, bone sialoprotein), bone sialoprotein, osteopontin, early T lymphocyte activation, osteopontin) [OPN]
Alleles: A1, A1, A2, B1, A2, B1, B2, B2
Insert Size (kb) 1.400
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Insert contains the following sites separated by (bp): (BamHI/BglII)- 272- AhaIII- 480- PvuII- 129- EcoRI- 209- XmnI- 116- HindIII- 61- SspI- 157- (BglII/BamHI). (BamHI and BglII sites destroyed in cloning.).
Restriction digests of the clone give the following sizes (kb): XbaI/PstI--2.75, 1.38; XbaI--4.0; PstI--4.0; EcoRI--3.5, 0.56; HindIII--3.6, 0.34.
The 5' EcoRI fragment of the clone detects genomic EcoRI fragments of 6.8 kb, 2.1 kb, and 0.9 kb in the human; 5.5 kb in the hamster; and 6.6 kb in the mouse. It also detects a human genomic BamHI fragment of 25 kb and KpnI fragment of 11.9 kb.
This probe is a full-length cDNA.
The human and rat polypeptide precursors are 65% identical. The cell-binding sequence and a potential N-linked glycosylation site are conserved in both human and rat.
Enzyme(s) not detecting polymorphism: EcoRI, HindIII, BamHI, XbaI, sacI, TaqI, PvuII, PstI, EcoRV, KpnI.
References

Kiefer MC, et al. The cDNA and derived amino acid sequence for human osteopontin. Nucleic Acids Res. 17: 3306, 1989. PubMed: 2726470

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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