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p13-1-25
p13-1-25
規(guī)格:
貨期:
編號(hào):B162633
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 p13-1-25
商品貨號(hào) B162633
Designations p13-1-25
Species Homo sapiens, human
Applications
Can use the entire plasmid as probe with standard hybridization and washing conditions.
Vector
Construct size (kb): 7.400000095367432
Insert
DNA: genomic
Insert lengths(kb): 2.0
Gene product: DNA Segment, single copy [D19S11]
Alleles: F1, F2, A1, A1, A2, A2, B1, B2, C1, C2, D1, D2, D3
Insert Size (kb) 2.0
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments
Restriction digests analyzed on agarose gels give the following sizes (kb): EcoRI--5.7, 1.2, 0.56, 0.28; Sau3A--1.7, 0.88, smaller fragments; HindIII: 7.4; PstI--5.0, 2.4; SalI--7.4.
EcoRI cuts the insert twice giving fragments of 1.2, 0.54 and 0.28 kb.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
This probe detects 3 different TaqI polymorphisms. Alpha (C alleles) is the same detected by p13-1-82 (ATCC 59004, 59005). Epsilon has more than 32 alleles. Phi (F alleles) has 2 common alleles. The combined heterozygosity for these is 98%.
The highly polymorphic epsilon polymorphism includes a relatively frequent null allele.
Can use the entire plasmid as probe with standard hybridization and washing conditions. Use a final wash of 0.1X SSC/0.1% SDS at 65C.
p13-1-82 (ATCC 59004, 59005), p13-2-31 (ATCC 59014, 59015), and p13-1-25 (ATCC 59870, 59871) were subcloned from cosmid 1-13 (C1-13).
References

Buroker NE, et al. A hypervariable region at the D9S11 locus. Hum. Genet. 76: 90-95, 1987. PubMed: 2883111

Michael Litt, personal communication

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