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MTC-M
MTC-M
規(guī)格:
貨期:
編號:B165203
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 MTC-M
商品貨號 B165203
Organism Mus musculus, mouse
Tissue thyroid
Cell Type C cell
Product Format frozen
Morphology epithelial
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease carcinoma
Age 20 months
Gender female
Strain BALB/c
Storage Conditions liquid nitrogen vapor phase
Derivation
This line was derived from a transplantable neoplasm of thyroid C cells.
Clinical Data
female
Genes Expressed
somatostatin; calcitonin
Cellular Products
somatostatin; calcitonin
Comments

The cells can be propagated in spinner cultures or in static cultures.

The amounts of stored and released somatostatin can be increased 2 to 3 fold by treatment for 4 days with 1 micromolar 5-bromo-2'-deoxyuridine.

Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 85%; heat-inactivated horse serum, 10%; fetal bovine serum, 5%
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 105 cells/mL and maintain between 1 x 105 and 3 x 106 cells/mL. Cell clumps should be dispersed using a small bore pipette.

Medium Renewal: Every 2 to 3 days

Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Population Doubling Time 96 hrs
Name of Depositor AS Tischler
Deposited As Mus musculus
References

Tischler AS, et al. Establishment of a continuous somatostatin-producing line of medullary thyroid carcinoma cells from BALB/c mice. J. Endocrinol. 110: 309-313, 1986. PubMed: 2875121

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

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