久久久久国产成人精品亚洲午夜,成人免费毛片一区二区三区,国产成人久久精品激情91,国产成人无码精品久久久免费,精品欧美国产一区二区三区不卡,国产成人一区二区三区影院,国产精品久久久久久,久久久WWW成人免费精品,欧美91精品久久久久网免费

熱門(mén)搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購(gòu)物車 1 種商品 - 共0元
當(dāng)前位置: 首頁(yè) > ATCC代理 > SW1463 [SW 1463, SW-1463]
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • [email protected]
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
SW1463 [SW 1463, SW-1463]
SW1463 [SW 1463, SW-1463]
規(guī)格:
貨期:
編號(hào):B165794
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 SW1463 [SW 1463, SW-1463]
商品貨號(hào) B165794
Organism Homo sapiens, human
Tissue rectum
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Dukes' type C, colorectal adenocarcinoma
Age 66 years
Gender female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Derivation
The line was derived from a grade II mucous producing and grade III solid tumor.
Clinical Data
66 years
Caucasian
female
Antigen Expression
blood type A; Rh +
Genes Expressed
carcinoembryonic antigen (CEA) 223 ng/106 cells/10 days; keratin
Cellular Products
carcinoembryonic antigen (CEA) 223 ng/106 cells/10 days; keratin
Tumorigenic Yes
Effects
Yes, in nude mice
Comments
CSAp negative (CSAp-).

The cells are positive for keratin by immunoperoxidase staining.

Tumor specific nuclear matrix proteins CC-4, CC-5 and CC-6 are expressed.

 

Complete Growth Medium The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)


Subculturing

Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

Subculture just prior to confluency

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.  Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mof complete growth medium and aspirate cells by gently pipetting. 
  5. To remove trypsin-EDTA solution, transfer cell suspension to the centrifuge tube and spin at approximately 125 x g for 5 to10 minutes.
  6. Discard supernatant and resuspend cells in fresh growth medium.  Add appropriate aliquots of cell suspension to new culture vessels.
  7. Incubate cultures at 37°C without CO2.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 1 to 2 times per week

Culture Conditions
Atmosphere: Air, 100%
STR Profile
Amelogenin: X
CSF1PO: 11,12
D13S317: 12,13
D16S539: 11
D5S818: 13,14
D7S820: 9
THO1: 6,7
TPOX: 8,11
vWA: 16
Isoenzymes
ES-D, 1
G6PD, B
PEP-D, 1
PGD, A
PGM1, 1
PGM3, 1-2
Name of Depositor A Leibovitz
Deposited As Homo sapiens
References

Leibovitz A, et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36: 4562-4569, 1976. PubMed: 1000501

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
溧阳市| 西充县| 庆安县| 屏山县| 黄梅县| 岳阳市| 会理县| 曲沃县| 天门市| 泸州市| 揭阳市| 开鲁县| 阳原县| 日照市| 西平县| 绥宁县| 元阳县| 鄯善县| 米林县| 手机| 浏阳市| 舒兰市| 紫阳县| 赞皇县| 汽车| 蒲城县| 德州市| 靖西县| 黔东| 固始县| 泰州市| 交口县| 贺兰县| 连江县| 贡山| 通州区| 大港区| 南木林县| 略阳县| 云南省| 汶川县|