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SW756
SW756
規(guī)格:
貨期:
編號:B165801
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 SW756
商品貨號 B165801
Organism Homo sapiens, human
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease squamous cell carcinoma
Age 46 years
Gender female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Karyotype modal number = 76; range = 60 to 96
Derivation
Temple Texas, United States
Clinical Data
female
Caucasian
46 years
Antigen Expression
HLA A1, A24, B8, B44, Cw2, Cx, DR6Y; Le3; Le4; Le5
Genes Expressed
HLA A1, A24, B8, B44, Cw2, Cx, DR6Y; Le3; Le4; Le5
Tumorigenic Yes
Effects
Yes, in nude mice
Virus Susceptibility Influenza A virus , Influenza A virus
Complete Growth Medium The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)


Subculturing
Protocol: Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 100%
Temperature: 37.0°C
STR Profile
Amelogenin: X
CSF1PO: 11,12
D13S317: 11
D16S539: 12,13
D5S818: 11,12
D7S820: 10,12
THO1: 9.3
TPOX: 8
vWA: 17
Isoenzymes
G6PD, B
GLO-I, 1-2
Name of Depositor Univ. Texas System, Board of Regents
Deposited As Homo sapiens
U.S. Patent Number
Year of Origin 1974
References

Freedman RS, et al. Monoclonal antibody to a human MDR1 multidrug resistance gene product, and uses. US Patent 5,434,076 dated Jul 18 1995

Freedman RS, et al. Characterization of a cell line (SW756) derived from a human squamous carcinoma of the uterine cervix. In Vitro 18: 719-726, 1982. PubMed: 7129487

PR8/A/34

The SW756 cell line was initiated by A. Leibovitz at the Scott and White Clinic, Temple, Texas in 1974 from biopsy material from a poorly differentiated invasive squamous carcinoma of the uterine cervix. The patient had not received chemotherapy or radiation prior to the biopsy.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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