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SW837 [SW-837]
SW837 [SW-837]
規(guī)格:
貨期:
編號(hào):B165802
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 SW837 [SW-837]
商品貨號(hào) B165802
Organism Homo sapiens, human
Tissue rectum
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease grade IV, adenocarcinoma
Age 53 years
Gender male
Ethnicity Caucasian
Applications
This cell line may be used as a transfection host.
Storage Conditions liquid nitrogen vapor phase
Clinical Data
53 years
Caucasian
male
Antigen Expression HLA A23, A32, B15, B35; blood type O; Rh+
Genes Expressed
carcinoembryonic antigen (CEA) 523 ng/10 6 cells/10 days; keratin
Cellular Products
carcinoembryonic antigen (CEA) 523 ng/10 exp6 cells/10 days; keratin
Tumorigenic Yes
Effects
Yes, in nude mice
Comments
The cells are negative for expression of CSAp (CSAp-) and colon antigen 3, negative.
There is a C -> T mutation in codon 248 of the p53 gene resulting in an Arg -> Trp substitution.

The cells are positive for keratin by immunoperoxidase staining.

Complete Growth Medium The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)


Subculturing

Remove medium, add fresh 0.25% trypsin, 0.02% EDTA for 10 to 20 minutes at 37°C. Add fresh medium, disperse cells and centrifuge to pellet the cells. Resuspend in fresh medium and dispense into new flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended

Medium Renewal: 1 to 2 times per week

Cryopreservation
Freeze medium: complete growth medium, 95%; DMSO, 5%
Culture Conditions
Atmosphere: air, 100%
STR Profile
Amelogenin: X
CSF1PO: 10
D13S317: 13
D16S539: 12
D5S818: 12
D7S820: 9,12
THO1: 9.3
TPOX: 8,9
vWA: 15,16
Isoenzymes
ES-D, 1
G6PD, B
PGD, A
PGM1, 1
PGM3, 1
Name of Depositor A Leibovitz
Deposited As Homo sapiens
References

Chen TR, et al. Intercellular karyotypic similarity in near-diploid cell lines of human tumor origins. Cancer Genet. Cytogenet. 10: 351-362, 1983. PubMed: 6652615

Chen TR, et al. Karyotype consistency in human colorectal carcinoma cell lines established in vitro. Cancer Genet. Cytogenet. 6: 93-117, 1982. PubMed: 7104989

Nigro JM, et al. Mutations in the p53 gene occur in diverse human tumour types. Nature 342: 705-707, 1989. PubMed: 2531845

Leibovitz A, et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36: 4562-4569, 1976. PubMed: 1000501

Rodrigues NR, et al. p53 mutations in colorectal cancer. Proc. Natl. Acad. Sci. USA 87: 7555-7559, 1990. PubMed: 1699228

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于經(jīng)理 18067160830 2088210172
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李經(jīng)理 13626845108 972239479
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