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The SWLA1 (ATCC HB-12559), SWLA2 (ATCC HB-12580), and SWLA3 (ATCC HB-12558) antibodies appear to recognize a species-specific lipooligosaccharide or lipopolysaccharide on the cell surface of S. mutans.

These antibodies can be used in the clinical diagnosis and treatment of dental caries in humans.

Spleen cells were fused with NS-1-Ag4-1 myeloma cells.

immunoglobulin; monoclonal antibody; against Streptococcus mutans

immunoglobulin; monoclonal antibody; against Streptococcus mutans

Animals were immunized with Streptococcus mutans Clarke (ATCC 25175).

Spleen cells were fused with NS-1-Ag4-1 myeloma cells.

The SWLA1 (ATCC HB-12559), SWLA2 (ATCC HB-12580), and SWLA3 (ATCC HB-12558) antibodies appear to recognize a species-specific lipooligosaccharide or lipopolysaccharide on the cell surface of S. mutans.

These antibodies have substantially no reactivity with any of the following bacterial strains: Streptococcus rattus (ATCC 19645, Streptococcus gordonii (ATCC 10558), Streptococcus gordonii (ATCC 13396), Streptococcus mitis (ATCC 49456).

Also Streptococcus sobrinus (ATCC 33478), Streptococcus sobrinus 6715, Streptococcus sanguis (ATCC 10556), Streptococcus sanguis (ATCC 49295), Streptococcus anginosus (ATCC 33397), Lactobacillus acidophilus (ATCC 4356), Lactobacillus casei (ATCC 4646),

Actinobacillus actinomycetemcomitans (ATCC 33384), Porphyromonas gingivalis (ATCC 33277), Prevotella intermedia (ATCC 49046), Bacteroides forsythus (ATCC 43047), Eikenella corrodens (ATCC 23834), Fusobacterium nucleatum (ATCC 25586),

Treponema denticola (ATCC 33520), Campylobacter rectus (ATCC 33238), Myxococcus xanthus DZ2, and Escherichia coli HB101.

These antibodies can be used in the clinical diagnosis and treatment of dental caries in humans.

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.

Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.

Note: About 50% of the cells may attach. Cells in suspension grow singly and in small clusters.

culture medium, 45%; fetal bovine serum, 45%; DMSO, 10%

Temperature: 37.0°C