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T27A
T27A
規(guī)格:
貨期:
編號(hào):B165814
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 T27A
商品貨號(hào) B165814
Organism Mus musculus, mouse
Tissue spleen
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease leukemia
Strain BALB/c
Applications
This line was derived from a leukemia induced by N-tropic Friend murine leukemia virus.
The cells are negative for surface immunoglobulin (sIg-) and Thy-1.2 (Thy-1.2-).
Tested and found negative for ectromelia virus (mousepox).
Storage Conditions liquid nitrogen vapor phase
Derivation
This line was derived from a leukemia induced by N-tropic Friend murine leukemia virus.
Antigen Expression
H-2d; Ia8
Genes Expressed
H-2d; Ia8
Tumorigenic Yes
Effects
Yes, in BALB/c mice
Comments
This line was derived from a leukemia induced by N-tropic Friend murine leukemia virus.
The cells are negative for surface immunoglobulin (sIg-) and Thy-1.2 (Thy-1.2-).
Erythroid differentiation and hemoglobin synthesis are NOT inducible by DMSO.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.01 mM 2-mercaptoethanol, 90%; heat-inactivated fetal bovine serum, 10%
Subculturing
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml.
The cells may also be grown as ascites in syngeneic mice. Inject 2 X 10(5) cell per mouse and harvest as soon as abdominal enlargement is observed (about 10 days).
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Name of Depositor B Chesebro
Deposited As Mus musculus
References

Chesebro B, et al. Lack of erythroid characteristics in Ia-positive leukemia cell lines induced by Friend murine leukemia virus: brief communication. J. Natl. Cancer Inst. 60: 239-242, 1978. PubMed: 564410

Chesebro B, et al. Characterization of Ia8 antigen, thy-1.2 antigen, complement receptors, and virus production in a group of murine virus-induced leukemia cell lines. J. Immunol. 117: 1267-1274, 1976. PubMed: 185295

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于經(jīng)理 18067160830 2088210172
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