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USASK/DSIL-LHRH-A1
USASK/DSIL-LHRH-A1
規(guī)格:
貨期:
編號(hào):B165914
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 USASK/DSIL-LHRH-A1
商品貨號(hào) B165914
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
It is recommended that the feeder cells be plated 24 hours before use at 2 X 10(6)/T75 in order to obtain a 30% confluent monolayer.
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against the carboxy terminal end of gonadotropin releasing hormone (also called luteinizing hormone releasing hormone (LHRH)
Cellular Products
immunoglobulin; monoclonal antibody; against the carboxy terminal end of gonadotropin releasing hormone (also called luteinizing hormone releasing hormone (LHRH)
Tumorigenic Yes
Effects
Yes, forms ascites in pristane primed BALB/c mice
Comments
Animals were immunized with LHRH conjugated to bovine serum albumin.
Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.
A feeder layer is required to recover the cells from a frozen ampule. Cells are grown at ATCC on 56-X feeder layer, irradiated STO cells. It is recommended that the feeder cells be plated 24 hours before use at 2 X 10(6)/T75 in order to obtain a 30% confluent monolayer.
Complete Growth Medium RPMI 1640 medium with 2 mM L-glutamine and 50 mg/l gentamicin, 70%; NCTC 109 medium, 10%; fetal bovine serum, 20%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. Cells are grown at ATCC on 56-X feeder layer, irradiated STO cells. It is recommended that the feeder cells be plated 24 hours before use at 2 X 10(6)/T75 in order to obtain a 30% confluent monolayer.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isotype IgG1
Name of Depositor University of Saskatchewan
Deposited As mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Silversides DW, et al. Monoclonal antibodies against LHRH: development and immunoactivity in vivo and in vitro. J. Reprod. Immunol. 7: 171-184, 1985. PubMed: 3884802

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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