久久久久国产成人精品亚洲午夜,成人免费毛片一区二区三区,国产成人久久精品激情91,国产成人无码精品久久久免费,精品欧美国产一区二区三区不卡,国产成人一区二区三区影院,国产精品久久久久久,久久久WWW成人免费精品,欧美91精品久久久久网免费

熱門(mén)搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購(gòu)物車(chē) 1 種商品 - 共0元
當(dāng)前位置: 首頁(yè) > ATCC代理 > Wgd5
▍最近瀏覽歷史
▍聯(lián)系我們
  • 0574-87157013
  • [email protected]
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
Wgd5
Wgd5
規(guī)格:
貨期:
編號(hào):B165946
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Wgd5
商品貨號(hào) B165946
Organism Mus musculus, mouse
Tissue connective tissue
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 2 Cells contain polyomavirus DNA sequences

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease Leukemia
Applications
transfection host
Helper cell line for packaging retroviral vectors such as those released from lambdaZd31, lambdaZd32, and lambdaZd35 (ATCC 37563-5, respectively).
Derivation
The line was derived from the WOP cell line (a 3T3 derivative transformed by an origin defective polyomavirus).
Genes Expressed
retrovirus vector (Moloney murine leukemia virus
Cellular Products
retrovirus vector (Moloney murine leukemia virus
Comments
The Wgd5 cell line contains a mutant of the Moloney murine leukemia virus (MoMuLV) that is useful for packaging recombinant retroviral DNA into infectious virus particles.
The line was derived from the WOP cell line (a 3T3 derivative transformed by an origin defective polyomavirus).
Wgd5 was selected as a gpt+ clone after cotransformation of WOP with pSV2gpt and a packaging mutant of MoMuLV.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium Dulbecco's modified Eagle's medium with 0.25 mg/ml xanthine, 0.015 mg/ml hypoxanthine, 0.01 mg/ml thymidine, 0.002 mg/ml aminopterin and 0.015 mg/ml mycophenolic acid, 90%; fetal bovine serum, 10%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, add fresh 0.25% trypsin, 0.02% EDTA solution for 1 to 2 minutes, rinse and remove trypsin.
Allow the cells to sit at 37C until they begin to detach (about 3 to 5 minutes), add fresh medium, aspirate and dispense into new flasks.
Subculture before the cells become confluent.
If allowed to become confluent, the cells will slough off and die.
Name of Depositor AJ Murphy
Deposited As Mus musculus
References

Murphy AJ, Efstratiadis A. Cloning vectors for expression of cDNA libraries in mammalian cells. Proc. Natl. Acad. Sci. USA 84: 8277-8281, 1987. PubMed: 3479791

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
罗田县| 新建县| 策勒县| 福州市| 额尔古纳市| 高唐县| 临海市| 永兴县| 凉城县| 陇川县| 平邑县| 汉川市| 鄂温| 兰州市| 夏邑县| 丽水市| 香格里拉县| 金寨县| 克东县| 体育| 东方市| 永善县| 湘乡市| 邛崃市| 新乡县| 子洲县| 乐业县| 定兴县| 井冈山市| 随州市| 普兰县| 浏阳市| 葫芦岛市| 嘉禾县| 玉树县| 安顺市| 津南区| 伊宁县| 东源县| 大埔县| 井陉县|