| 產(chǎn)品名稱 |
X16C8.5 [X16C] |
| 商品貨號(hào) |
B165964 |
| Organism |
Mus musculus, mouse |
| Cell Type |
B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
reticulum cell sarcoma |
| Strain |
BALB/cAnN |
| Applications |
Derived from a spontaneous reticulum cell neoplasm found in an old BALB/cAnN mouse. Tested and found negative for ectromelia virus (mousepox). |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
near tetraploid; modal number = 78; range = 39 to 84 |
| Derivation |
Derived from a spontaneous reticulum cell neoplasm found in an old BALB/cAnN mouse. |
| Antigen Expression |
I-A+; H-2d |
| Receptor Expression |
Fc |
| Genes Expressed |
immunoglobulin (both surface and secreted, sIg+),I-A+; H-2d,Expression of I-A and H-2 is increased by treatment with interferon. |
| Cellular Products |
immunoglobulin (both surface and secreted, sIg+) |
| Tumorigenic |
No |
| Effects |
No, The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium. |
| Comments |
Derived from a spontaneous reticulum cell neoplasm found in an old BALB/cAnN mouse. Expression of I-A and H-2 is increased by treatment with interferon. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
RPMI 1640 medium with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. |
| Cryopreservation |
culture medium 95%; DMSO, 5% |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Isotype |
IgM |
| Population Doubling Time |
18 hrs |
| Name of Depositor |
KJ Kim |
| Deposited As |
Mus musculus |
| References |
Kim KJ, et al. Establishment and characterization of BALB/c lymphoma lines with B cell properties. J. Immunol. 122: 549-554, 1979. PubMed: 310843
Kim KJ, et al. Characterization of T-cell-soluble factors modulating the expression of Ia and H-2 antigens on BALB/c B lymphoma cell lines. Cell. Immunol. 76: 253-267, 1983. PubMed: 6404559
|
