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XMMME-001
XMMME-001
規(guī)格:
貨期:
編號(hào):B165972
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 XMMME-001
商品貨號(hào) B165972
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
When cultures are seeded at 5 X 10(4) cells/ml, antibody production may not be detectable until days 6 or 7.
Storage Conditions liquid nitrogen vapor phase
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with P3X63Ag8 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human melanoma tumor specific antigen
Cellular Products
immunoglobulin; monoclonal antibody; against human melanoma tumor specific antigen
Comments
When cultures are seeded at 5 X 10(4) cells/ml, antibody production may not be detectable until days 6 or 7.
Animals were immunized with M21 human melanoma cells.
Spleen cells were fused with P3X63Ag8 myeloma cells.
Complete Growth Medium The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
  • Subculturing
    Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10(4) cells/ml and maintain between 1 X 10(5) and 2 X 10(6) cells/ml.
    Medium Renewal: Every 2 to 3 days
    Cryopreservation
    Freeze medium: culture medium, 95%; DMSO, 5%
    Storage temperature: liquid nitrogen vapor phase
    Isotype IgG2a
    Name of Depositor Xoma Corporation
    Deposited As mouse (B cell); mouse (myeloma)
    U.S. Patent Number
    References

    Scannon PJ, et al. Human melanoma specific immunotoxins. US Patent 4,590,071 dated May 20 1986

    Levy JA, et al. Purification of monoclonal antibodies from mouse ascites eliminates contaminating infectious mouse type C viruses and nucleic acids. Clin. Exp. Immunol. 56: 114-120, 1984. PubMed: 6201307

    梅經(jīng)理 17280875617 1438578920
    胡經(jīng)理 13345964880 2438244627
    周經(jīng)理 17757487661 1296385441
    于經(jīng)理 18067160830 2088210172
    沈經(jīng)理 19548299266 2662369050
    李經(jīng)理 13626845108 972239479
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