久久久久国产成人精品亚洲午夜,成人免费毛片一区二区三区,国产成人久久精品激情91,国产成人无码精品久久久免费,精品欧美国产一区二区三区不卡,国产成人一区二区三区影院,国产精品久久久久久,久久久WWW成人免费精品,欧美91精品久久久久网免费

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > XR-V15B
▍最近瀏覽歷史
▍聯(lián)系我們
  • 0574-87157013
  • [email protected]
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
XR-V15B
XR-V15B
規(guī)格:
貨期:
編號:B165975
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 XR-V15B
商品貨號 B165975
Organism Cricetulus griseus, hamster, Chinese
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Derivation
XR-V15B is a X-ray sensitive Chinese Hamster Lung fibroblast cell line isolated from an ethylnitrosourea (ENU)-mutagenized cell population of V79 cells on the basis of its hypersensitivity to X-rays.
Comments
XR-V15B is a X-ray sensitive Chinese Hamster Lung fibroblast cell line isolated from an ethylnitrosourea (ENU)-mutagenized cell population of V79 cells on the basis of its hypersensitivity to X-rays.
These cells belong to X-ray complementation group 5 and are mutant in the p86 subunit of the Ku autoantigen.
Ku has been shown to be involved in DNA double-strand break repair and V(D)J recombination. Cells will revert to wild type on treatment with azacytidine.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:8 to 1:12 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, rinse quickly with 0.25% trypsin, 0.03% EDTA solution, add 1-2 ml additional trypsin solution and allow flasks to set at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new flasks.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor G Chu
References

Rathmell WK, Chu G. Involvement of the Ku autoantigen in the cellular response to DNA double-strand breaks. Proc. Natl. Acad. Sci. USA 91: 7623-7627, 1994. PubMed: 8052631

Errami A, et al. Ku86 defines the genetic defect and restores X-ray resistance and V(D)J recombination to complementation group 5 hamster cell mutants. Mol. Cell. Biol. 16: 1519-1526, 1996. PubMed: 8657125

Smider V, et al. Restoration of X-ray resistance and V(D)J recombination in mutant cells by Ku cDNA. Science 266: 288-291, 1994. PubMed: 7939667

Zdzienicka MZ, et al. Characterization of an X-ray-hypersensitive mutant of V79 Chinese hamster cells. Mutat. Res. 194: 239-249, 1988. PubMed: 2460764

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
鞍山市| 西畴县| 吴江市| 津市市| 慈溪市| 南川市| 柳林县| 巴林左旗| 棋牌| 土默特右旗| 巴彦淖尔市| 吕梁市| 廉江市| 长宁县| 湖南省| 普陀区| 尖扎县| 同德县| 平原县| 房产| 邛崃市| 盐源县| 依兰县| 宽城| 万全县| 焉耆| 普定县| 阿克| 龙川县| 临海市| 万荣县| 科尔| 德庆县| 渑池县| 新邵县| 莫力| 桐梓县| 杂多县| 琼结县| 隆回县| 都兰县|