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Y-3P
Y-3P
規(guī)格:
貨期:
編號(hào):B165984
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Y-3P
商品貨號(hào) B165984
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with I-A b, f, p, q, r, s, u, and v haplotypes and weakly with I-A k.
It cross-reacts with all rat I-A like molecules.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against I-A; cytotoxic
Cellular Products
immunoglobulin; monoclonal antibody; against I-A; cytotoxic
Tumorigenic Yes
Effects
Yes, in BALB/c mice
Comments
Animals were immunized with spleen cells from (BALB/c X C57BL/6)F1 mice and boosted with spleen cells from BALB/c mice immunized with either BALB.B or C57BL/6 T cell blasts.
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody reacts with I-A b, f, p, q, r, s, u, and v haplotypes and weakly with I-A k.
It cross-reacts with all rat I-A like molecules.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
Culture Medium: RPMI medium 1640 with 10 mM HEPES, 95%; fetal bovine serum, 5%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Culture Conditions
Temperature: 37.0°C
Isotype IgG2a
Name of Depositor C Janeway
Deposited As mouse (B cell); mouse (myeloma)
References

Janeway CA Jr., et al. Monoclonal antibodies specific for Ia glycoproteins raised by immunization with activated T cells: possible role of T cellbound Ia antigens as targets of immunoregulatory T cells. J. Immunol. 132: 662-667, 1984. PubMed: 6228596

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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