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YI 328-18
YI 328-18
規(guī)格:
貨期:
編號:B165988
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 YI 328-18
商品貨號 B165988
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain: ?BALB/c? (B cell); BALB/c (myeloma)

Applications
Both YI328-18 (ATCC HB-9306)and YI328-51 (ATCC HB-9307) specifically recognize the abnormal branching structure of the lactoganglioseries glycolipid antigen molecule and have almost no cross-reactivity with smaller unbranched segments.
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with P3X63Ag8U.1 mouse myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against glycosphingolipid
Cellular Products
immunoglobulin; monoclonal antibody; against glycosphingolipid
Comments
Animals were immunized with a synthetic lactoganglioseries glycolipid.
Spleen cells were fused with P3X63Ag8U.1 mouse myeloma cells.
Both YI328-18 (ATCC HB-9306)and YI328-51 (ATCC HB-9307) specifically recognize the abnormal branching structure of the lactoganglioseries glycolipid antigen molecule and have almost no cross-reactivity with smaller unbranched segments.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 days
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Cryopreservation
Freeze medium: complete growth medium supplemented with an additional 10% fetal bovine serum and 10% (v/v) DMSO.
Culture Conditions
Temperature: 37.0°C
Isotype IgG3
Name of Depositor R Kannagi
U.S. Patent Number
References

Kannagi R, et al. Monoclonal antibodies specific for synthetic glycosphingolipids having abnormal branched structure. US Patent 4,885,358 dated Dec 5 1989

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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