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<i>Clostridioides difficile</i> (Prevot) Lawson et al.
<i>Clostridioides difficile</i> (Prevot) Lawson et al.
規(guī)格:
貨期:
編號(hào):B166172
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Clostridioides difficile (Prevot) Lawson et al.
商品貨號(hào) B166172
Deposited As Clostridium difficile (Hall and O'Toole) Prevot
Strain Designations VPI 10463
Application
Enteric Research
Isolation
Abdominal wound
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol&reg; no
Genotype Toxinotype: 0
Images Micrograph of Cells at 1000X ATCC 43255
Type Strain no
Comments
Presence of tcdA and tcdB genes confirmed by PCR.
Toxinotype 0
Binary toxin gene cdtB not amplified by PCR
Ribotype 087
Toxin B production confirmed by cytotoxicity assay using Vero cells (Cell line # CCL-81).
Medium ATCC® Medium 2107: Modified Reinforced Clostridial
ATCC® Medium 260: Trypticase soy agar/broth with defibrinated sheep blood
Growth Conditions
Temperature: 37°C
Atmosphere: Anaerobic
Name of Depositor TD Wilkins, DM Lyerly
Chain of Custody
ATCC <-- TD Wilkins, DM Lyerly <-- G Nygaard
References

Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303

BD GeneOhm Cdiff. BD Becton Dickinson.

Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999

Fawley WN, Wilcox MH; on behalf of the Clostridium difficile Ribotyping Network for England and N. Ireland (CDRN). An enhanced DNA fingerprinting service to investigate potential Clostridium difficile infection case clusters sharing the same PCR-ribotype. J. Clin. Microbiol. 49(12): 4333-4337, 2011. PubMed: 21956986

Kink JA, Williams JA. Antibodies to recombinant Clostridium difficile toxins A and B are an effective treatment and prevent relapse of C. difficile-associated disease in a hamster model of infection. Infect. Immun. 66: 2018-2025, 1998. PubMed: 9573084

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