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hp2alpha [hp2-alpha]
hp2alpha [hp2-alpha]
規(guī)格:
貨期:
編號:B226632
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 hp2alpha [hp2-alpha]
商品貨號 B226632
Designations hp2alpha [hp2-alpha]
GenBank Number

X00606

Species Homo sapiens, human
Applications
A small HindIII/PstI linker was used in the process of inserting the PstI/BamHI fragment into the HindIII and BamHI sites of pAT153.
Vector
Construct size (kb): 3.799999952316284
Insert
DNA: cDNA
Insert lengths(kb): 0.5
Tissue: adult
Gene product: haptoglobin [HP]
Alleles: B1, B2, A1, A1, A2, A2, C1, C2
Insert Size (kb) 0.5
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments
Restriction digests of the clone give the following sizes (kb): BamHI/HindIII--3.25, 0.6; BamHI--3.8; PstI--2.8, 0.71; HindIII--3.8; EcoRI--3.8.
The insert covers the entire alpha chain coding region and leader. There are typically two copies of the gene per haploid genome.
HindIII detects a 1.7 kb length polymorphism which is also detected by BamHI with fragment sizes of 7.5 kb and 9.2 kb.
The HindIII polymorphism is also detected by AvaII with fragment sizes of 4.4 kb (freq = 0.65) and 2.7 (freq = 0.35). The AvaII RFLP also appears to be in linkage equilibrium with a TaqI RFLP at the CETP locus.
There is a polymorphic EcoRI site about 4 kb 3' to Hp2 which reduces the allele size to 10.1 (indistinguishable from the Hp1 allele).
For the XbaI polymorphism, the 5.8 kb constant band is from the HPR sequences, the 4.6 kb band is from the Hp2 or Hp1F alleles, and the 4.0 kb band is from the Hp1S allele.
For the HindIII polymorphism, the 3.4 kb constant band is from the HPR sequences, the 4.9 kb band is from the Hp2 allele, and the 3.2 kb band is from the Hp1S or Hp1F alleles.
For the EcoRI polymorphism, the 8.3 kb constant band is from the HPR sequences, the 11.8 kb band is from the Hp2 allele, and the 10.1 kb band is from the Hp1S or Hp1F alleles.
A small HindIII/PstI linker was used in the process of inserting the PstI/BamHI fragment into the HindIII and BamHI sites of pAT153.
References

Bashir A, et al. An AvaII polymorphism in the haptoglobin alpha gene (HPA). Nucleic Acids Res. 17: 4906, 1989. PubMed: 2568614

Radice P, et al. Loss of heterozygosity in human germinal tumors. Cytogenet. Cell Genet. 52: 72-76, 1989. PubMed: 2612217

Maeda N, et al. Duplication within the haptoglobin HP2 gene. Nature 309: 131-135, 1984. PubMed: 6325933

Nobuyo Maeda, personal communication

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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