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p304
p304
規(guī)格:
貨期:
編號(hào):B226800
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) p304
商品貨號(hào) B226800
Designations p304
GenBank Number

J00127

Species Homo sapiens, human
Depositors ST Lord
Applications
The depositor notes that the fibrinogen clones (ATCC 59706-59710) are suitable for applications in gene mapping or involving nucleic acid hybridization, but they are not suitable starting material for expression in mammalian cells.
Vector
Construct size (kb): 4.400000095367432
Insert
DNA: cDNA
Insert lengths(kb): 2.0
Tissue: liver
Gene product: fibrinogen, A alpha polypeptide [FGA]
Alleles: A1, A2
Insert Size (kb) 2.0
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.2, 1.9, 0.4; XhoI--4.8; EcoRI/XhoI--2.1, 1.1, 0.98; PstI--3.3, 1.1; BglI--3.2, 1.2; EcoRI/HindIII--2.3, 1.9; NcoI--2.5, 1.9.
Insert encodes fibrinogen A alpha amino acids 1-625 with an initiating ATG in front of amino acid 1. The vector is an expression vector.
The depositor notes that the fibrinogen clones (ATCC 59706-59710) are suitable for applications in gene mapping or involving nucleic acid hybridization, but they are not suitable starting material for expression in mammalian cells.
The clones do not encode the signal peptide necessary for assembly and secretion and one is known to lack a single nucleotide in the coding region.
The ability of this probe to detect the TaqI polymorphism was verified by J. Murray, University of Iowa.
References

Chung DW, et al. Characterization of complementary deoxyribonucleic acid and genomic deoxyribonucleic acid for the beta-chain of human fibronogen. Biochemistry 22: 3244-3250, 1983. PubMed: 6688356

Lord ST. Expression of a cloned human fibrinogen cDNA in Escherichia coli: synthesis of an A alpha polypeptide. DNA 4: 33-38, 1985. PubMed: 3882374

Susan T Lord, personal communication

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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