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pRB394
pRB394
規(guī)格:
貨期:
編號(hào):B226933
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pRB394
商品貨號(hào) B226933
Designations pRB394
Depositors R Bruckner
Biosafety Level 1
Vector Information
Size (kb): 6.5000000000000000
Vector: pRB394 (plasmid)
Construction: pUB110, pBR322, cat
Marker(s):ampR,bleR,neoR
Construct size (kb): 6.5
Features: insert detection: CAT
marker(s): ampR, neoR, bleR
replicon: pUB110, pMB1
terminator: rrnB, to phage lambd
Applications
promoter-cloning vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--6.7; BamHI--6.7; BglI/BglII--3.5, 3.2.
Structural stability of the plasmid in B. subtilis can be affected by high levels of protein production. Under these conditions, cell growth and stability may be improved by reducing the antibiotic concentration in the media.
May not be suitable for cloning very strong expression signals.
Promoter-cloning shuttle vector using the expression of chloramphenicol acetyltransferase (CAT) as the reporter. Also useful for construction of fusion proteins.
Neo confers resistance to neomycin and kanamycin and ble confers resistance to bleomycin and phleomycin.
The cat gene was derived from pUB112 by deletion of the promoter and the regulatory inverted repeat, resulting in constitutive cat gene expression when provided with an appropriate upstream promoter.
The order of the major features in the plasmid is: To terminator - HindIII/MCS/EcoRI - cat - rrnB terminator - ampR - pMB1 ori - pUB110 ori - neoR - bleR.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Bruckner R. A series of shuttle vectors for Bacillus subtilis and Eschericia coli. Gene 122: 187-192, 1992. PubMed: 1452028

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