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pB1.4 hum [pB1.4.hum]
pB1.4 hum [pB1.4.hum]
規(guī)格:
貨期:
編號:B227076
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pB1.4 hum [pB1.4.hum]
商品貨號 B227076
Designations pB1.4 hum [pB1.4.hum]
GenBank Number

X52882

Species Homo sapiens, human
Depositors KR Willison
Vector
Construct size (kb): 3.299999952316284
Insert
DNA: cDNA
Insert lengths(kb): 0.5799999833106995
Gene product: t-complex 1(T-complex protein 1) [TCP1]
Alleles: A1, A2
Insert Size (kb) 0.580
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.65, 0.6; BamHI--3.3; PstI--3.3; HindIII--3.3.
There are 4 TCP1-related sequences/haploid genome--1 expressed and 3 pseudogenes. One pseudogene maps to 7p14-cen, another may to 5. The expressed sequence shows most homology to mouse; all human sequences are more similar than to mouse.
Insert encodes 3' sequences. Shows about 90% homology with mouse sequences and determines an amino acid sequence showing about 98% homology with mouse protein.
The mouse and human TCP1 ORFs both encode polypeptides of 556 amino acids which are 95.5% homologous.
Detects mRNAs of 1.7 kb (major) and 2.4 kb (minor). Differences are apparently due to polyadenylation.
References

Fonatsch C, et al. Assignment of the TCP1 locus to the long arm of human chromosome 6 by in situ hybridization. Cytogenet. Cell Genet. 45: 109-112, 1987. PubMed: 3476253

Willison K, et al. Chromosomal assignment of TCP-1 the human homologue of a mouse T-complex locus. Cytogenet. Cell Genet. 40: 779-780, 1985.

Kirchhoff C, Willison K. Nucleotide and amino-acid sequence of human testis-derived TCP1. Nucleic Acids Res. 18: 4247, 1990. PubMed: 2377466

Willison K, et al. The human homologue of the mouse T-complex gene, TCP1, is located on chromosome 6 but is not near the HLA region. EMBO J. 6: 1967-1974, 1987. PubMed: 3653076

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