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pHEBo1 [pHEBo]
pHEBo1 [pHEBo]
規(guī)格:
貨期:
編號(hào):B227154
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pHEBo1 [pHEBo]
商品貨號(hào) B227154
Designations pHEBo1 [pHEBo]
Depositors Wisconsin Alumni Res. Fndn., HW Bremer, Wisconsin Alumni Res. Fndn.
U.S. Patent
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Biosafety Level 1
Host
Distribution host: Escherichia coli
Vector Information
Size (kb): 6.9000000953674320
Vector: pHEBo1 (plasmid)
Promoters: Promoter HSV TK
Construction: EBV, HSV-1, pBR322
Marker(s):ampR,hygR
Construct size (kb): 6.900000095367432
Features: marker(s): ampR, hygR
promoter: HSV TK
replicon: pMB1, EBV oriP
Applications
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.9; ClaI--6.9; EcoRI--4.4, 2.3; EcoRV--4.6, 2.4; HindIII--6.9; SalI--6.9.
A map is provided in this reference.
Shuttle vector that can replicate and be selected in Escherichia coli and mammalian cells.
The order of the major features in this plasmid is: EcoRI - ClaI - HindIII - BamHI - SalI - oriP - HSV-1 tk polyadenylation - <- hygR - HSV-1 tk promoter - < - ampR.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Sugden WM. Recombinant vector and eukaryotic host transformed thereby. US Patent 4,686,186 dated Aug 11 1987

. Molecular cloning: a laboratory manual. Cold Spring Harbor, NY: CSHL Press; 1989.

Shipped freeze-dried
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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