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Diplonema papillatum (Porter) Triemer and Ott
Diplonema papillatum (Porter) Triemer and Ott
規(guī)格:
貨期:
編號(hào):B227234
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Diplonema papillatum (Porter) Triemer and Ott
商品貨號(hào) B227234
Deposited As Isonema papillatum Porter
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
seawater on the surface of eel grass, Zostera marina, New Hampshire, 1985
Product Format frozen
Type Strain no
Medium ATCC® Medium 1532: Isonema medium
ATCC® Medium 1532: Isonema medium
ATCC® Medium 1728: Enriched Isonema medium
Growth Conditions
Temperature: 25.0°C
Duration: axenic
Cryopreservation

1.?? Harvest the culture by agitating the contents of each flask.? Transfer the cell suspensions to 15 ml plastic centrifuge tubes.

2.?? Spin the cell suspensions at approximately 500 x g for 5 min.

3.?? Pool the cell pellets and adjust the concentration to 2.0 - 4.0 x 107 cells/ml with a fresh ATCC medium 1728.

????? *If the concentration is too low centrifuge at 500 x g for 5 min and resuspend in the volume of ATCC medium 1728 required to yield the desired concentration.

4.?? Mix the cell preparation and 20% (v/v) DMSO in equal portions.? The final concentration will be 1.0 - 2.0 x 107 cells/ml and 10% DMSO.? The time from the mixing of the cell preparation and cryoprotective solution to the start of the freezing process should be no less than 15 min. and no more than 30 min.

5.?? Dispense in 0.5 ml aliquots to 1.0-2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation).

6.?? Place the vials in a controlled rate freezing unit.? From room temperature cool at -1°C/min to -40°C.? If the freezing unit can compensate for the heat of fusion, maintain rate at??????? -1°C/min through the heat of fusion.? At -40°C plunge into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.? Place the apparatus at -80°C for 2.5 to 3 hours and then plunge ampules into liquid nitrogen.? (The cooling rate in this apparatus is approximately

-1°C/min.) ?

7.?? Store in either the vapor or liquid phase of a nitrogen refrigerator.

8.?? To thaw a frozen ampule, place it in a 35°C water bath such that the lip of the ampule remains above the water line. Thawing time is approximately 2 to 3 minutes.? Do not agitate the ampule.? Do not leave ampule in water bath after thawed.

9.?? Immediately after thawing, aseptically transfer contents to a T-25 tissue culture flask containing 10 ml ATCC medium 1728.

10.????????? Incubate in a 25°C incubator with the cap screwed on tightly.

?

Name of Depositor PA Kivic
Special Collection NSF - Protistology
Chain of Custody
ATCC <<--PA Kivic<<--D. Porter
Year of Origin 1985
References

van Leeuwen F, et al. beta-D-glucosyl-hydroxymethyluracil is a conserved DNA modification in kinetoplastid protozoans and is abundant in their telomeres. Proc. Natl. Acad. Sci. USA 95: 2366-2371, 1998. PubMed: 9482891

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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