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pPR5
pPR5
規(guī)格:
貨期:
編號:B227509
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pPR5
商品貨號 B227509
Designations pPR5
Species Escherichia coli (Migula) Castellani and Chalmers
Depositors OmniGene, Inc., KM Overbye, OmniGene, Inc.
Applications
produces protein alkaline phosphatase
Vector
Construct size (kb): 5.900000095367432
Insert
DNA: genomic
Insert lengths(kb): 3.200000047683716
Gene product: alkaline phosphatase [phoA]
Target Gene: alkaline phosphatase
Insert Size (kb) 3.200
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--3.2, 2.7; SmaI--5.9; BamHI--5.7, 0.2; XhoI--5.9.
Construct permitting expression and secretion of alkaline phosphatase using the chitinase promoter and signal sequences.
The insert contains the complete coding sequence for phoA, as well as the induction signals, ribosome-binding site and the signal sequence for S. plicatus chitinase-56 (also called chitinase-63). The preferred cleavage site is after Ala30.
A HindIII digest yields a 3.2 kb fragment, containing approximately 250 bp of Streptomyces DNA (the chitinase regulatory sequences), and coding for a chitinase-phoA fusion protein.
The phoA sequences can be removed vy a SmaI/XhoI digest and replaced with other sequences to be expressed and secreted.
Classification Enterobacteriaceae, Escherichia
References

Overbye KM, et al. DNA encoding a Streptomyces endochitinase 56 signal peptide. US Patent 5,188,961 dated Feb 23 1993

Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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