久久久久国产成人精品亚洲午夜,成人免费毛片一区二区三区,国产成人久久精品激情91,国产成人无码精品久久久免费,精品欧美国产一区二区三区不卡,国产成人一区二区三区影院,国产精品久久久久久,久久久WWW成人免费精品,欧美91精品久久久久网免费

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > p5-1-25 [p1-25]
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • [email protected]
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
p5-1-25 [p1-25]
p5-1-25 [p1-25]
規(guī)格:
貨期:
編號:B227748
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 p5-1-25 [p1-25]
商品貨號 B227748
Designations p5-1-25 [p1-25]
Species Homo sapiens, human
Applications
The most efficient detection of RFLPs at the D2S3 locus is by use of the cosmid 1-5 subclones p5-1-25 (ATCC 59684) and p5-2-96 (ATCC 59000).
Vector
Construct size (kb): 8.600000381469727
Insert
DNA: genomic
Insert lengths(kb): 3.200000047683716
Gene product: DNA Segment, single copy [D2S3]
Alleles: C4, J10, A1, B1, B2, C1, C2, C3, D2, G4, J1, J3, J4, J5, J6, J7, J8, K2, A2, D1, E1, E2, G1, G2, G3, J2, J9, K1
Insert Size (kb) 3.200
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--5.4, 3.2; HindIII--8.6; EcoRI/HindIII--5.4, 3.2; PstI--6.0, 1.8, 0.88.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
p5-1-25 is a single copy subclone from cosmid 1-5.
The most efficient detection of RFLPs at the D2S3 locus is by use of the cosmid 1-5 subclones p5-1-25 (ATCC 59684) and p5-2-96 (ATCC 59000). Use of p5-1-30 (ATCC 59006) and p5-1-32 (ATCC 59002) probably adds little to the PIC.
Subclone 5-1-32 detects the PstI insert/deletion RFLP with TaqI but bands are larger and difficult to resolve.
Long runs on 20 cm long 1% agarose gels, such that all fragments less than about 1.5 kb have run off the end, are required to obtain adequate resolution.
References

Litt M. A single-copy subclone, p1-25, from cosmid 1-5, defines a highly polymorphic RFLP on 2q35->37 [HGM8 no. D2S3]. Nucleic Acids Res. 14: 4378, 1986. PubMed: 3714483

Litt M, et al. A highly polymorphic locus in human DNA revealed by probes from cosmid 1-5 maps to chromosome 2q35-37. Am. J. Hum. Genet. 38: 288-296, 1986. PubMed: 3006480

Lathrop GM, et al. Three genetic linkage groups on chromosome 2. Cytogenet. Cell Genet. 46: 644, 1987.

Asher JH Jr., et al. Waardenburg syndrome (WS): the analysis of a single family with a WS1 mutation showing linkage to RFLP markers on human chromosome 2q. Am. J. Hum. Genet. 48: 43-52, 1991. PubMed: 1670751

Litt M, White RL. A highly polymorphic locus in human DNA revealed by cosmid-derived probes. Proc. Natl. Acad. Sci. USA 82: 6206-6210, 1985. PubMed: 2994065

Michael Litt, personal communication

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
新化县| 襄垣县| 石首市| 吴旗县| 怀柔区| 蒙山县| 当涂县| 台湾省| 安吉县| 新闻| 高唐县| 阿鲁科尔沁旗| 通渭县| 洛扎县| 白朗县| 苍南县| 大安市| 皋兰县| 泾阳县| 广丰县| 乌兰察布市| 团风县| 藁城市| 高安市| 东方市| 晋宁县| 衡阳县| 昌江| 汝州市| 滨州市| 鸡东县| 玉林市| 恩施市| 库伦旗| 夹江县| 万载县| 扬州市| 当雄县| 茶陵县| 平利县| 马尔康县|