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Size (kb): 6.3000001907348630

Vector: pcos1EMBL (cosmid)

Promoters: Promoter none

Construction: R6K, pcos4EMBL

Marker(s):kanR,tetR

Construct size (kb): 6.300000190734863

Features: marker(s): kanR, tetR
promoter: none
replicon: R6K
terminator: none
enhancer: none

vector for constructing cDNA libraries

Restriction digests of the clone give the following sizes (kb): BamHI--6.3; SalI--6.3.

IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.

One of a family of cosmid vectors (ATCC 37568-37571) containing a R6K origin.

Contains one lambda cos site.

Temperature: 30.0°C