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phIL1AcDNA [human IL-1alpha cDNA]
phIL1AcDNA [human IL-1alpha cDNA]
規(guī)格:
貨期:
編號:B240500
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱 phIL1AcDNA [human IL-1alpha cDNA]
商品貨號 B240500
Designations phIL1AcDNA [human IL-1alpha cDNA]
GenBank Number

M28983

Species Homo sapiens, human
Depositors U Gubler
Vector
Construct size (kb): 6.400000095367432
Insert
DNA: cDNA
Insert lengths(kb): 2.400000095367432
Tissue: peripheral blood cells (LPS activated)
Gene product: interleukin 1, alpha [IL1]
Insert Size (kb) 2.400
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
The insert contains the following restriction sites (nt positions according to the published sequence): EcoRI--434; HindIII--227; ScaI--1670.
Restriction digests of the clone give the following sizes (kb): BamHI--4.0, 2.4; EcoRI--4.2, 2.2; HindIII--4.1, 2.3; SacI-- uncut; PstI--6.4.
Contains the complete coding sequence (nt 38-852).
pMG-5 is a pBR322 derivative in which the sequence adjacent to the EcoRV site has been modified (GGATATCG to GGATATCC) to allow generation of flanking BamHI sites after homopolymeric tailing EcoRV-cut vector with C's.
References

Gubler U, et al. Recombinant human interleukin 1 alpha: purification and biological characterization. J. Immunol. 136: 2492-2497, 1986. PubMed: 3485152

Ueli Gubler, personal communication

梅經理 17280875617 1438578920
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李經理 13626845108 972239479
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