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<i>Clostridioides difficile</i> (Prevot) Lawson et al.
<i>Clostridioides difficile</i> (Prevot) Lawson et al.
規(guī)格:
貨期:
編號(hào):B240758
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Clostridioides difficile (Prevot) Lawson et al.
商品貨號(hào) B240758
Deposited As Clostridium difficile (Hall and O'Toole) Prevot
Strain Designations 5283
Application
Enteric Research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Preceptrol&reg; no
Genotype Toxinotype: 0 RefRupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999
Type Strain no
Comments
Restriction endonuclease (REA) typing performed by the lab of D Gerding indicates that this strain is type K3. Types belonging to REA Group "K" have been identified as toxinotype 0 with standard pathogenicity locus genes.
Presence of tcdA and tcdB genes confirmed by PCR.
Binary toxin gene cdtB not amplified by PCR
Toxinotype 0
Ribotype 053
PFGE type Nap5
REA type K3
Depositor-provided information: PFGE Type NAP5, Toxinotype 0, and Binary Toxin negative.
Medium ATCC® Medium 2107: Modified Reinforced Clostridial
ATCC® Medium 260: Trypticase soy agar/broth with defibrinated sheep blood
Growth Conditions
Temperature: 37°C
Atmosphere: Anaerobic
Name of Depositor B Limbago
References

Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303

Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999

Fawley WN, Wilcox MH; on behalf of the Clostridium difficile Ribotyping Network for England and N. Ireland (CDRN). An enhanced DNA fingerprinting service to investigate potential Clostridium difficile infection case clusters sharing the same PCR-ribotype. J. Clin. Microbiol. 49(12): 4333-4337, 2011. PubMed: 21956986

Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999

Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999

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