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Human adenovirus 4
Human adenovirus 4
規(guī)格:
貨期:
編號:B241080
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Human adenovirus 4
商品貨號 B241080
Classification Adenoviridae, Mastadenovirus, Human adenovirus E
Agent Human adenovirus 4
Strain RI-67
Applications
Respiratory research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments Derived by adaptation of HEK cell product ATCC® VR-1081™ to HeLa-229 cells (ATCC® CCL-2.1™). ATCC® VR-1081™ and ATCC® VR-1572™ have not been compared for sequence or infectivity in primary cell culture and tissue culture.

HEK cells are known to contain Human adenovirus 5 DNA, and Human adenovirus 5 has been detected by PCR in this item.
Effect on Host CPE, cell rounding and degeneration
Recommended Host HeLa-229 (ATCC® CCL-2.1™)
Growth Conditions Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 70-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 3-7 days
Name of Depositor WP Rowe, National Institute of Allergy and Infectious Diseases; ATCC®
Special Collection ATCC
Source Throat washings of patient, Fort Leonard Wood, Missouri, 1952-1953
References

Huebner RJ, et al. Adenoidal-pharyngeal-conjunctival agents: a newly recognized group of common viruses of the respiratory system. N. Engl. J. Med. 251: 1077-1086, 1954. PubMed: 13223958

Werner JH, Hilleman MR. Recovery of new agent from patients with acute respiratory illness. Proc. Soc. Exp. Biol. Med. 85: 183-188, 1954. PubMed: 13134329

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