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VC85
VC85
規(guī)格:
貨期:
編號(hào):B242189
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 VC85
商品貨號(hào) B242189
Designations VC85
Species Homo sapiens, human
Vector
Construct size (kb): 4.800000190734863
Insert
DNA: genomic
Insert lengths(kb): 2.099999904632568
Gene product: DNA Segment, single copy [D1S85]
Alleles: A1, A2
Insert Size (kb) 2.100
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 2.1; BamHI--4.8; PstI--3.3, 1.5; SmaI--4.8; HindIII--4.8.
D1S85 has been shown to be tightly linked to D1S86.
This probe is a subclone of the 16.41 kb probe VC85.
The 2.1 kb sequence in the insert is from the smaller EcoRI allele. The other allele is 2.6 kb.
The allele frequencies given were determined in a Caucasian population. Distinct frequencies have been determined in Negro and Bushman (San) populations.
This probe detects a 500 bp insertion/deletion polymorphism which can be detected with BglII, StuI, PvuII, PstI, EcoRI, HindIII, and BamHI. Because TaqI and MspI cut within this fragment, allele sizes do not vary by 500 bp.
Enzyme(s) not detecting polymorphism: HindII, SstI, AvaII, RsaI, MboI.
References

Verga V, et al. An arbitrary single copy DNA sequence VC85 (D1S85) detects a 500 bp insertion/deletion polymorphism on chromosome 1. Nucleic Acids Res. 17: 4007, 1989. PubMed: 2567507

Dracopoli NC, et al. The CEPH consortium linkage map of human chromosome 1. Genomics 9: 686-700, 1991. PubMed: 2037294

Vera Verga, personal communication

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